Abstract

HELMINTHOLYTIC ACTIVITY OF LEAVES OF FENUGREEK PLANT (TRIGONELLA FOENUM-GRAECUM)

Prapulla Putta*

Abstract

The aim of the present study was to evaluate helmintholytic activity of crude methanolic and aqueous extract of leaves of Trigonella Foenumgraecum using Pheretima posthuma as test worm. Various concentrations of (25, 50, 100 mg/ml) of methanolic and aqueous extracts were tested in the bioassay, which involved the determination of time of paralysis (P) and time of death (D) of the worms. Albendazole was included as standard reference and distilled water as control. Aqueous and methanolic extracts displayed significant Helmintholytic activity at highest concentration of 100 mg/ml. The extracts of leaves of Trigonella Foenum-graecum had shown a dose dependant inhibition of spontaneous motility (Paralysis) of earthworms. The gradual increased in a dose exhibited a gradual increase in the activity. The results of present study indicated that the crude aqueous extract significantly paralysis and also caused death of worm in short time, as compared to methanolic extract. In conclusion, the traditional use of leaves of the plant Trigonella Foenum-graecum as an helmintholytic activity have been confirmed and further studies are suggested to isolate the active principles responsible for activity.

Keywords: Albendazole, Helmintholytic, Pheretima posthuma, Trigonella Foenumgraecum.