Abstract

SODIUM DODECYL SULPHATE POLYACRYLAMIDE GEL ELECTROPHORESIS (SDS-PAGE) PATTERN OF PRE-ANTI RETROVIRAL THERAPY(PRE ART) AND ANTI RETROVIRAL THERAPY(ART) TREATED MALE AND FEMALE HIV/AIDS PATIENTS

S.Vijayalakshmi, Dr.C.Chandran, Dr.MP.Saravanan, Dr.S.Dhanapaul

Abstract

Human immunodeficiency virus (HIV) causes Acquired immune deficiency syndrome. Human immune system is mainly affected by HIV. HIV transmitted through anal, vaginal or oral sex, blood transfusion, contaminated needles, exchange between mother and baby during pregnancy, breastfeeding, or other exposure to one of the above bodily fluids. Currently no vaccine is available for AIDS and HIV. Antiretroviral treatment reduces the mortality and the morbidity of HIV infection. HIV positive serum was collected from 5 group of patients. CD4 count was measured using flowcytometry. Serum proteins were separated by SDS – PAGE. In the SDS – PAGE, Alpha1 antitrypsin, Pre albumin, Albumin, Transferrin, Beta zone and Gamma zone was observed.3 year after the treatment of ART protein levels are increased in the male samples.

Keywords: HIV, serum protein, ART, CD4 count.