Abstract

PHYTOCHEMICAL SCREENING AND GC-MS ANALYSIS OF METHANOL EXTRACT OF THE LEAVES OF IPOMOEA CARNEA

G. B. Alaka Kar*, Dr. Susanta Kumar Rout and Debashisa Mishra

Abstract

Ipomoea carnea is one of the medicinally important plants belonging to the family Convolvulaceae has been studied for different biological activities with special reference to Central Nervous System (CNS) disorders. The experimental results showed that Ipomoea carnea has effective in various CNS disorders along with woundhealing and anti inflammatory activity. Methanol extract of Ipomoea carnea at 400mg/kg showed highly significant result towards different animal models mimicking the different CNS disorders. Similarly the sub maximal dose i.e. 200 mg/kg showed significant effect in different animal models. Different literature study revealed that the plant possess antimicrobial, anticancer, anti-inflammatory activity. I. carnea which was introduced into some regions as an ornamental plant, is a globally distributed invasive shrub found in tropical and subtropical regions. Preliminary qualitative phytochemical screening of I. carnea revealed the presence of phenolic compounds, terpenoids, flavonoids and steroids. Some of them have antioxidant and antimicrobial activities. GC-MS analysis in the leaf powder’s methanol extract was done by using National Institute Standard and Technology (NIST) database 2005 to identify the compounds present. The spectra of unknown compounds were compared with that of known compounds stored in the NIST library by matching the molecular weight and retention time. 22 bio active phytochemical compounds were identified in leaf powder. The compounds predominantly Phenolic compounds, Flavonoids derivatives, Carbohydrate, Glycoside, Saponin, Phytosterols on basis of the molecular formula, molecular weight, peak area percentage etc. These different active phytochemicals have been found to possess a wide range of activities, which may help in the protection against different diseases.

Keywords: Phytochemicals, GCMS analysis, spectra, antibacterial activity.