Abstract

DEVELOPMENT AND VALIDATION OF LC-MS/MS METHOD FOR THE SIMULTANEOUS DETERMINATION OF ΒETA-SITOSTEROL, THYMOL AND OLEANOLIC ACID IN LEUCAS ASPERA LINN

Prashant Hande*, Ajitkumarbs, Shailendra Rane, Manish Hate and Jalil K. Shaikh

Abstract

A simple, precise and accurate LC-MS/MS method has been developed for simultaneous determination of beta-sitosterol, thymol and oleanolic acid in whole plant powder of Leucas aspera linn. MRM (Multiple Reaction Monitoring) transitions 397.20>161.20, 151.10>109.10 and 455.40>455.55 were optimized on Shimadzu triple quadrupole mass spectrometer instrument (Model: LCMS-8040) for quantification of beta-sitosterol, thymol and oleanolic acid respectively. Chromatographic method was developed on a Shimadzu shimpack-XR C8 column (100mm x 2.0mm x 2μ) using gradient program. The proposed method was validated for linearity, accuracy, precision, and recovery, limit of detection (LOD) and limit of quantitation (LOQ). The validated LCMS/ MS method can be used for a routine quality control analysis and simultaneous quantitation of beta-sitosterol, thymol and oleanolic acid in Leucas aspera linn.

Keywords: LC-MS/MS, Beta-sitosterol, Thymol, Oleanolic acid, Leucas aspera linn., Validation.