Abstract

EVALUATION OF HEPATOPROTECTIVE ACTIVITY OF LEAF EXTRACT OF PHYLLANTHUS EMBLICA AND MORINGA OLEIFERA ON CARBON TETRACHLORIDE INDUCED HEPATIC DAMAGE IN ALBINO RATS

Malliga Elangovan*, M. S. Dhanarajan and I. Elangovan

Abstract

Phyllanthus emblica and Moringa oleifera have been used in Ayurveda for treatment of hepatic disorders. Most of the pharmacological studies are largely focused on their fruits while the rest of the parts remain less investigated. Therefore, we aimed to investigate the hepatoprotective effect of the petroleum ether extract of Phyllanthus emblica (PELPE) and Moringa oleifera (PELMO) leafs on carbon tetrachloride (CCl4) induced hepatotoxicity model in Albino rats. 54 male albino rats were divided into nine groups. Mice orally received saline, 100, 200, or 300 mg/kg b.w of PELPE /PELMO, 300 mg/kg b.w of PELPE+ PELMO respectively for fourteen days. Treatment groups were challenged with single dose of 3 ml/kg b.w of CCl4. Exposure of CCl4 elicited severe reduction in serum antioxidant enzymes. Hepatoprotection of PELPE and PELMO are evidenced by restoration of ALT, AST, Total protein, Superoxide dismutase, Catalase, Glutathione peroxidase and further confirmed by Liver histopathological studies. Presence of α–Tocopherol, β-Amyrin of M. oleifera and octadecanoic acid, methyl ester, gamma sitosterol of P emblica may be responsible for the bioactivity of these extracts. PELPE and PELMO possess significant hepatoprotective activities against CCl4 induced hepatotoxicity in rats and the extracts might be effective in management of liver damage.

Keywords: Antioxidants, CCl4, Hepato protective activity, Phyllanthus emblica, Moringa oleifera, and Histopathology.