Abstract

COMPARATIVE STUDY OF RASAPARPATI- AN AYURVEDIC FORMULATION ON INTESTINAL MOTILITY AND ITS AMELIORATIVE EFFECTS ON MUCOSAL DAMAGE IN RAT MODEL OF ULCERATIVE COLITIS

Dr. Mona Mashru*, Dr. Rajesh Baravaliya and Dr. Mita Mashru

Abstract

Background: Ayurvedic classics claim the usefulness of Rasaparpati in Grahani diseaeses. As per modern view, Grahani is equated with malabsoption syndrome, disturbed intestinal motility, chronic colitis etc. Various methods described for Shodhana (purification and/or detoxification) of Parada (mercury) used as one of the ingredients in the preparation Rasaparpati. Therefore, in the present study three different formulations of Rasaparpati prepared by SamanyaSodhita, Astasamskarita and AnuvasitaParada and evaluated for its efficacy on intestinal motility and ameliorative effects in modulating the extent and severity of experimental ulcerative colitis in rats. Materials & methods: Effect of Rasaparpati samples on intestinal motility was checked through latency of onset of kaolin expulsion through GI tract in mice. Test drugs treated mice received respective sample of Rasaparpati (32.5 mg/kg, po). The anti-colitis activity was assessed through acetic acid-induced experimental colitis in Charle’s foster rats. Test drugs were administered for 7 consecutive days to respective groups in dose of 22.5 mg/kg body weight of rats. Colitis was induced by intra-colonic instillation of 5 ml/kg of 4% (v/v) acetic acid in rats. The inflammatory response was assessed by effect on severity of ulceration and histopathological study. Result: Rasaparpati prepared by AstasamskaritaParada produced increase in intestinal motility and fecal output in mice. Rasaparpati prepared by Astasamskarita and AnuvasitaParada administrated groups produced an apparent decrease in the area and severity of ulceration in experimental colitis in rats. However, it was found to be statistically non-significant. Rasaparpati prepared by AstasamskaritaParada administered group produced better result in acetic acid-induced ulcerative colitis. Conclusion: These findings reveal the effects of Rasaparpati prepared by AstasamskaritaParada in intestinal motility and down regulation of inflammatory response in acetic acid-induced colitis in rats.

Keywords: Grahani, Intestinal motility, Parada, Rasaparpati, Ulcerative colitis.