Abstract

EVALUATION OF ANTIOXIDANT ACTIVITY OF METHANOLIC EXTRACT OF LEAVES OF LEUCAS ZEYLANICA LINN BY USING ISOLATED FROG HEART

Swetha Bindu CH.*

Abstract

The present study was aimed for the induction of oxidative stress by using H2O2 on isolated frog heart and evaluates the antioxidant activity of methanolic extract of leaves of Leucas zeylanica Linn. When frog ringer solution containing 1mM of H2O2 perfused to frog heart preparation, which indicating the induction of oxidative stress on frog heart by H2O2 solution, this might be due to desensitization of receptors. By using H2O2, shows negative ionotropic and chronotopic effects and the cardiac arrest was produced at 20th minute. This result supports the frog heart model for induction of oxidative stress by H2O2. In the presence of methanolic extract of leaves of Leucas zeylanica, the cardiac arrest was observed at 38th minutes i.e. heart was protected longer period that indicates antioxidant activity which was compared with the standard ascorbic acid. In the presence of ascorbic acid cardiac arrest was observed at 40th minute.

Keywords: Frog heart, antioxidant activity, oxidative stress Leucas zeylanica, methanolic extract.