Abstract

ISOLATION AND IDENTIFICATION OF CHEMICAL CONSTITUENTS FROM VARIOUS POLAR SOLVENT CRUDE LEAF, STEM AND ROOT EXTRACTS OF ENDEMIC - POGOSTEMON SPECIOSUS BENTH OF THE NILGIRI

S. Jeyaraman*, S. Rajeshkumar, M. C. Nisha, K. Kalimuthu and Chinnadurai Vajjiram

Abstract

Pogostemon speciosus Benth is endemic ethno medicinal plant,belongs to the family Lamiaceae found in Anamalai and Nilgiri hills ofSouth Western Ghats, India. The present study was to analysis thesecondary metabolites by preliminary phytochemical screening of theleaf (PSLPE, PSLEA, and PSLE), stem (PSSPE, PSSEA, PSSE) androot (PSRPE, PSREA, PSRE) petroleum ether, ethyl acetate andethanol extracts by different test. Identification of the phytocompoundthrough FTIR and GCMS from leaf, stem and root ethanol extracts(PSLE, PSSE, PSRE). Among these extracts, the ethanol extracts ofleaf, stem and root showed the presence of majority of the metaboliteswhen compared to other extracts. FTIR analysis of PSLE, PSSE andPSRE revealed the presence of 6, 5, 7 major bonds respectively. The results pertaining toGCMS analysis led to the identification of number of compounds from the ethanol leaf, stemand root extracts. GCMS examination revealed 40, 29 and 29 phytocompounds separately,with 7, 7 and 4 bioactive compounds. The important bioactive compounds present in PSLEare Caryophyllene, 1-Naphthalenol, Alpha.-bisabolol, N-Hexadecanoic acid, Phytol, Hexatriacontane, 2-Tetracosahexaene, Dotriacontane, and Butyl-4(adamantyl-1) benzene, in PSSE extract Caryophyllene, Alpha.-bisabolol, 2H Pyran, Flopropione, Phytol isomer and Dotriacontane. The bioactive compounds present in PSRE extract are Caryophyllene, Heneicosane, 2H Pyran, Dotriacontane with anti-inflammatory, antifungal, antioxidant, antitumor, antibacterial, anti-asthmatics, drugs for skeletal disorders, bronchodilators, antispasmodic, hypocholesterolemic, nematicide, pesticide, anti-inflammatory, cytotoxic bioactivity.

Keywords: Pogostemon speciosus, potassium bromide, triterpenoids, alpha bisabolol.