Abstract

TRANSPORT SYSTEM FOR L-METHIONINE IN CORYNEBACTERIUM GLUTAMICUM X300

Subhadeep Ganguly and Kunja Bihari Satapathy

Abstract

The transport of L-methionine by a multiple analogue-resistant mutant Corynebacterium glutamicumX300 was studied. Only one transport system with low affinity (Km=15.1nmols-1g-1) for L-methionine was found in this mutant. Influx of L-methionine into the cells was accelerated by Na+ and K+. The effect of other metal ions was almost negligible. Effect of L-methionine analogues on L-methionine uptake by this mutant was also investigated. The effect was comparatively mild as it was a multiple analogue resistant strain. Different metabolic inhibitors studied exhibit significant inhibitory role on L-methionine uptake by the mutant. Uptake of L-methionine by NEM_untreated cells potentiated the efflux of L-methionine by the mutant.

Keywords: Influx, Efflux, L-methionine , Mutant.