Abstract

CHARACTERIZATION OF CAMPESTEROL, STIGMASTEROL AND ?-SITOSTEROL FROM VINCA ROSEA LEAVES BY GC-MS AND BIOLOGICAL EVALUATION

Gangadhar A. Meshram, Shraddha S. Khamkar, Ganesh S. Metangale,
Tanushri S. Kulkarni

Abstract

Vinca rosea, perennial shrub, grows throughout India. The species has long been cultivated and used for various treatments like diabetes mellitus, high blood pressure and infection. Various fractions of leaves extract of vinca rosea were evaluated to study the effect on glucoamylase, in vitro. Amongst the fractions, Petroleum ether: Chloroform (50:50) showed crystalline component and further, GCMSrevealed it comprised of campesterol, stigmasterol and β-sitoterol. It is observed that the fraction in total was moderate activator of glucoamylase with 51.87% activation. We report that this fraction would be used in the treatment of hypoglycemia and chronic diarrhea caused due to deficiency of glucoamylase in children.

Keywords: Vinca rosea, glucoamylase, activator.