Abstract

AN INDIRECT SPECTROPHOTOMETRIC DETERMINATION OF FUROSEMIDE IN PHARMACEUTICAL PREPARATIONS

Farha Khalaf Omar* and Hind Shaker Mahmod

Abstract

A new, simple, sensitive indirect spectrophotometric method for determination of Furosemide has been developing. The method involved addition of a known excess of sodium hypochlorite to in acidic medium, followed by determination of residual sodium hypochlorite by reacting with a fixed amount of methyl red, measuring the absorbance at 521nm. In this method the amount of sodium hypochlorite reacted correspond to the amount of Furosemide and the measured absorbance was found to increase linearly with the concentration of Furosemide, which is corroborated by the correlation coefficient of 0.9996. Beer's law plot showed a good Correlation in the concentration rang 1- 18 μg mL -1. The apparent molar absorptivity were calculated to be1.2 ×104l.mol-1. cm-1. The limit of detection (LOD) and quantification (LOQ) were calculated to be 0.019 and 0.0527μg mL-1. The relative standard deviation (RSD) was less than 1.7% (n=5). The method was applied to the determination of furosemide in pharmaceutical preparations (tablets and injection).

Keywords: Furosemide, Spectrophotometry, Pharmaceutical preparations.