Abstract

AQUACULTURE OF SHRIMP: WITH REFERENCE TO PCR DIAGNOSIS OF WSSV IN THE EGGS OF PENAEUS INDICUS

P. Karnan* and Dr. R. Usha

Abstract

Aquaculture, an important technique practiced in India and all over the world is the cultivation of not only fishes but also other valuable aquatic organisms such as prawns, algae, crabs, lobsters, shell fishes and pearls. The infection of WSSV in the eggs of P.indicus is diagnosed by a biotechnological tool PCR. The tests conducted from November to January show less than 40% of infection with 2 positive results of 5 tests undertaken. From March, the percentage of WSSV infection is slowly increased (March 50%, April 67%) and reaches the peak of more than 80% in May and June. This percentage is then decreased in July 67% August and September 50% and October shows less than 40% of infection (33%). This Variation in percentage of WSSV infection is mainly depended the temperature. Fluctuation due to high temperature May and June shows high percentage of infection, July to November show average value of WSSV infection and December and January show very low percentage of infection due to lesser temperature than other months. Hence it is clear that the infection of WSSV from the broadstock to larval stages is through the genome of the eggs in P.indicus. It is believed that the transmission of WSSV from the brooders to the larvae may be by the vertical transmission through the genome of the eggs.

Keywords: WSSV, PCR, R-PCR, DNTP, Bp, cDNA, P.indicus, Transmission, Life Cycle.