Abstract

EVALUATION OF HEPATOPROTECTIVE ACTIVITY OF POLYHERBAL (HEPIN) AGAINST ALCOHOL INDUCED HEPATOTOXICITY IN WISTAR ALBINO RATS

*Sherin Joseph, Dr. Abdul Vahab A., Dr. Jyoti Harindran and Dr. R Shankar Iyer

Abstract

Objectives: To investigate hepatoprotective activity of polyherbal formulation in alcohol induced hepatotoxicity in rats. Methods: Hepatotoxicity was induced in albino Wistar rats by oral administration of 40% ethanol (2ml/100g body weight). Polyherbal formulation “Hepin” was administered at a dose level of 250 mg/kg and 500mg/kg orally for 28 days. On the next day blood was taken by cardiac puncture and used for estimation of biochemical parameters like SGPT, SGOT, ALP, Bilirubin, Total Protein. Animals were sacrificed by cervical dislocation and liver was dissected out and exvivo studies and histopathological analysis of liver was carried out. Results: Obtained results revealed that administration of ethanol caused a significant increase in plasma SGPT, SGOT, ALP and Bilirubin compared to the control group. While total protein concentration is significantly declined. Additionally, a significant decrease in the level of hepatic GSH was also observed. However, the treatment of experimental rats with Hepin prevented these alterations and maintained the antioxidant status. The histopathological observations supported the biochemical evidences of hepatoprotection. Conclusions: The results of the present investigation indicate that Hepin possesses hepatoprotective activity.

Keywords: Hepatoprotective, Polyherbal, Hepin.