Abstract

DIFFERENTIATION AND AUTHENTICATION OF MEDICINAL ORCHIDS OF HABENARIA SPECIES USING RAPD- A MOLECULAR PROFILE

B. N. Satish and Mallya Suma V.*

Abstract

Introduction: Habenaria longicorniculata J.Graham medicinal orchid with multiple therapeutic usages also found adulterated with some Ashtavarga drugs. Authentic quality parameters of a therapeutic agent are most essential part in any science. Hence an attempt to generate DNA based molecular marker, RAPD. Materials and Methods: Habenaria longicorniculata J.Graham along with the other two species ie Habenaria crinifera Lindl., and Habenaria plantaginea Lindl., were selected for study. Leaf samples of these three orchids were used for RAPD. Genomic DNA isolation was carried out through CTAB method and purified. This was randomly amplified through PCR using different primers. Result: Five random primers were used OPD-02, OPB – 08, OPAI -05, OPC-07 and OPZ – 11 and the sequence obtained respectively was GGACCCAACC, GTCCACACGG, GTCGTAGCGG, GTCCCGACGA and CTCAGTCGCA. These can be used to differentiate and authenticate study sample.

Keywords: Habenaria longicorniculata J. Graham, RAPD, DNA, Primer.