Abstract

EVALUATION OF ANTI-CANCER ACTIVITY OF VASAGULUCHYADI KASHAYAM ON DEN AND PHENOBARBITONE INDUCED HEPATOCELLUAR CARCINOMA IN MALE RATS

*M. Sanjay, G. Ariharasivakumar, K. T. Manisenthil Kumar, M. Thanga Kokila, V. Sanish Devan and A. Jeevanantham

Abstract

The objective of this study is to find the chemoprotective effect of Vasaguluchyadi Kashayam in DEN and PHENOBARBITONE induced hepatocarcinogenesis in male Sprague Dawley rats. The important steps involved selection, constituent Analysis, in-vivo and in-vitro antioxidant activity. Initiation and promotion of Hepatocellular carcinoma (HCC) was done by single i.p injection of DEN at a dose of 200mg/kg and phenobarbitone 0.05% was given through orally in drinking water. The standard [5-flurouracil (20mg/kg) i.p] and formulation treatment were given after 14 days of development of HCC. The results showed that the injection DEN and PHENOBARBITONE (through drinking water) lead to the development of liver tumors in rats. In disease control group, serum biochemical and haemotological parameters such as SGOT, SGPT, ALP, urea, total bilirubin, total protein and creatinin levels were increased, and RBC, WBC and Hb levels were decreased when compared to normal group. In disease control group SOD, CAT, GSH levels were decreased and LPO level was increased. The result revealed that formulation treatment (Preventive) group gives excellent shielding against HCC and produced all the parameter in near normal range with a maintained antioxidant enzyme system. The results obtained showed that formulation was found to containing phenols at a concentration of 52.06 mg/g and flavonoids 67.84 mg/g. In the DPPH, ABTS, FRAP and phosphomolybdenum assay formulation has produced the highest antiradical activity and was also comparable with the standard quercetin The present study reveal the efficacy of the Formulation to prevent malignancy induced by chemical carcinogen and the constituents responsible for activity.

Keywords: DPPH, ABTS, FRAP and phosphomolybdenum.