Abstract

AN INVITRO SCREENING OF ANTI MICROBIAL ACTIVITY OF SELECTED DISINFECTANTS AGAINST SELECTED MICROBIAL STRAINS

Vuchooru Gayathri*, Sruthi Prathapan, Raja Kotagiri, Ravi Kumar Yerram, Naresh Suserla

Abstract

The antimicrobial agents that are used to inhibit or prevent the growth of microbes on inanimate objects and floors: ideally killing of 99.999% are termed as disinfectants. The efficacy is a key attribute to a disinfectant for its successful application which depends on several factors. This disinfectant efficacy is demonstrated predominantly by means of use dilution method and hard surface carrier test in any pharmaceutical facility. Use dilution method can be employed to screen the disinfectants in terms of concentration and contact time, upon challenging them against certain selected microorganisms including environmental isolates from that pharmaceutical facility. Hereby, this paper emphasizes on performing the use dilution method to define the efficacy of commercially available disinfectants namely Protasan, Combatan and Novocide against targeted microorganisms listed in table-2. These three disinfectants displayed identical effective concentration of 2.5% and same contact time of 10 minutes on all the targeted microorganisms for a notable 3 log reduction in the microbial population. These outcomes paved a way for demonstrating the applicable concentration and contact time of a suitable disinfectant on a regular basis in a pharmaceutical facility which have to be further confirmed by the hard surface carrier test for satisfying its intended application on different types of surfaces in a pharmaceutical facility.

Keywords: Antimicrobial agents classification, Mode of action of disinfectants, Disinfectant efficacy testing, Use dilution method.