Abstract

SCREENING OF ISOLATED PHYTOSTEROL FROM LEAVES OF HOLOPTELEA INTEGRIFOLIA (ROXB) PLANCH FOR ANTIOXIDANT ACTIVITY

Ranjana Sharma, Sudhir Singh Gangwar, Amita Tilak, R. N. Thakur and Ravindra C. Sutar*

Abstract

Holoptelea integrifolia (Roxb.) Planch has been used from long time in traditional medicine. The main objective of the work was to evaluate the antioxidant activity of Holoptelea integrifolia isolated Phytosterol (HIIP).The antioxidant activity of HIIP isolated from petroleum ether extract of leaves was evaluated using Total Reduction Capability. Preliminary Phytochemical investigation of the petroleum ether extract (PEHI) of Holoptelea integrifolia leaves reveals the presence of steroids, terpenoids, alkaloids, glycosides, flavonoids, proteins, tannins, and carbohydrate. In case of Total Reduction Capability HIIP showed dose dependent increase in absorbance thereby dose dependant total Reduction capacity indicating antioxidant activity. The results confirms that HIIP from petroleum ether extract also shows antioxidant activity using Total Reduction Capability, which suggests the application of the plant as an antioxidant agent.

Keywords: Holoptelea integrifoila isolated phytosterol, Antioxidant, Total Reduction Capability.