Abstract

ISOLATION AND PURIFICATION OF COLLAGEN FROM MARINE SPONGE DENDRILLA MEMBRANOSA

Jency George*, Joicy Abraham, Mahija S. P. and Dr. Manjusha W. A.

Abstract

Collagen is a protein and is widely used in medical applications. Collagen plays an important role in the formation of tissues and organs, and is involved in various functional expressions of cells. Nowadays collagen is isolated from calf, porcine tissue etc. As an alternative, collagen can be isolated from marine sponge. The aim of the present work is to be isolated and partially characterized the collagen from the marine sponge, Dendrilla membranosa. The isolated collagen was estimated by using Braford assay and partially purified by using gel filtration on a Sepharose 4B column. These purified collagens were conformed by SDS PAGE. The isolated material was dispersed in the following solvents at room temperature to investigate their solvent action: acetone, methanol, ethanol 96%, ethyl acetate, double-distilled water, citric acid and hydrochloric acid 37%. Now a days commercially available collagen in the market are expensive and are not affordable to the common people. The present information on collagens from marine sponge Dendrilla membranosa easily isolated and could assist the future attempts to unravel the therapeutically important, safer collagens from marine sponges for their use in biomedical field.

Keywords: Collagen, Dendrilla membranosa, SDS PAGE, gel filtration.