Abstract

ANTIOXIDANT AND CYTOTOXIC ACTIVITY AND POLYPHENOLS ANALYSIS USING HPLC-DAD - MS/MS OF BETULA RADDEANA GROWING IN GEORGIA

Zardiashvili L.*, Jokhadze M., Kuchukhidze J., Chincharadze D., Bojhadze A., Sivsivadze K.

Abstract

According to modern pharmacological study the genus Betula has choleretic, diuretic, antibacterial, antifungal, antioxidant and antiinflammatory effects. Species of the genus Betula that grow in Georgia are not sufficiently studied from the point of view of Pharmacognosy. The qualitative and quantitative composition and activity of the polyphenols sum, their factions and individual substances is not determined. It should be noted that there is great interest among scientists in the search for natural compounds with antioxidant and cytotoxic effect. Studies have shown that the main pharmacological a role of polyphenols is in the treatment of cardiovascular diseases and malignant tumors. Based on the presented above, the authors have carried out the fractionation of polyphenols of Betula raddeana using solvents of different polarity by liquid-liquid extraction. The cytotoxic and antioxidant effects of the methanol extract and the organic fractions were studied in vitro with the DPPH and WST-1 reagent respectively. Methanol extract (LC50 8.5 ± 0.1 μg / ml) and ethyl acetate fractions (LC50 9.8 ± 0.1 μg / ml) have a high cytotoxic activity. Their antioxidant activity is (IC50 0.29 ± 0.02 mg / ml) and (IC50 0.34 ± 0.02 mg / ml), respectively. This is due to a high content of polyphenolic compounds.

Keywords: Betula, cytotoxic and antioxidant action, polyphenol, LC-DAD-MS/MS.