Abstract

HYDROGEN PEROXIDE INDUCED DNA DAMAGE: PROTECTION BY ANTIOXIDANT COLEUS AROMATICUS

Dinesha Ramadas, Sachidananda Gurumahadevaiah., Harsha Ramakrishna, Subhas Chandrappa Mundasada

Abstract

The antioxidant properties of boiling water extract of Coleus aromaticus were evaluated using lipid peroxidation inhibition activity, hydroxyl radicals scavenging activity, 1,1-diphenyl-2-picryhydrazyl (DPPH) radical scavenging effect and Superoxide radical scavenging activity. The extracts of Coleus aromaticus effectively protects H2O2 (100μM) induced cell death in human peripheral lymphocytes. Further Coleus aromaticus extract prevents H2O2 (1mM) mediated calf thymus DNA damage as evidenced by agarose gel electrophoresis. The present study reveals that, the boiling water extract of Coleus aromaticus showed significant antioxidant and DNA damage protectant activity when compared to antioxidants like BHA, Curcumin and α-tocopherol.

Keywords: Antioxidants, Coleus aromaticus, Cytotoxicity, DNA damage, H2O2.