Abstract

DETERMINATION OF PHYTOCONSTITUENTS & EVALUATION OF ANTIOXIDANT, ANGIOGENIC AND ANTIMICROBIAL ACTIVITY OF ELYTRARIA ACAULIS (L. FIL.) LINDAU.

Vinoth M.* and Vijaya C.

Abstract

Objective: Elytraria acaulis easily available plant and used to treat various diseases in traditional medicine, such as wound healing, leucorrhoea, abscesses etc... Antioxidant, Angiogenic and Antimicrobial activity is essential for wound healing with this background this study presently focused to specifically determining the phytochemical, Antioxidant, angiogenic activity and antimicrobial activity of Elytraria acaulis. Materials & Methods: Alcoholic Extract of Elytraria acaulis (l. fil.) Lindau. (AEEA) subjected to Phytochemical Screening Test, HPTLC analysis, Determination of Total Phenolic & Flavonoid contents. Anti-oxidant Activity by Nitric oxide Scavenging Assay, Reducing Power assay, Lipid Peroxidation assay. Angiogenesis Activity Screened by CAM Models. Anti-microbial studies performed by Contact Bioautography. Results: Total Flavonoid & Phenolic content of AEEA was found to be 57.2 ± 0.26 mg Quercetin Equivalents / g 217.5 ± 0.5 mg Gallic acid equivalents /g plant extract respectively. In Nitric oxide Scavenging Assay. IC50 value of Standard was found to be 12.58 μg/ ml and in that of AEEA is 294.18± 11.25 μg/ ml. Reducing Power assay the observation shows reducing power of our drug (AEEA) is 75% compared to standard drug (100%). In Lipid Peroxidation assay IC50 value of Standard found to be 23.71± 2.12 μg/ ml and AEEA was 226.18± 4.04 μg/ ml. Angiogenic effect AEEA treated (500μg) group showed higher number of conspicuous blood vessels with greater thickness compared to control group. Contact Bioautographic antimicrobial study zone of inhibition correlated into HPTLC Analysis. Conclusion: The results of the present study conform AEEA have antioxidant, angiogenic and antimicrobial activity.

Keywords: Antioxidant, Angiogenic, Bioautography-Antimicrobial Activity, Elytraria acaulis.