Abstract

ASSESSMENT OF CIPROFLOXACIN CONTENT VARIATION IN ANTIMICROBIAL SUSCEPTIBILITY TEST DISCS BY LIQUID CHROMATOGRAPHY

Mahathi Kothapalli*, Gurubasavaraja swamy PM, V.N.Raju Erumalla, Rajesh Telugu, Swetha Chenna

Abstract

A new simple, rapid, selective, precise and accurate isocratic reverse phase high performance liquid chromatography assay has been developed for the estimation of Ciprofloxacin bulk powderand in discs. The separation was achieved by using C-18 column (Phenomenex Luna C18 column 250 mm×4.6mm id, 5μ particle size) coupled with a guard column of silica in mobile phase ammonium acetate buffer and acetonitrile (25mM ammonium acetate buffer with 75:25). The flow rate was 1.0ml/min and the drug was detected using UV detector at the wavelength of 275nm. The retention time was within 2.468 - 2.473 minutes. The method was validated as per ICH guidelines. Antimicrobial activity viz., Minimum inhibitory concentration (MIC) and the Minimum bactericidal concentration (MBC) against S. aureus and E. coli was subjected by using ciprofloxacin bulk powder and discs, and the results were interpreted by determining the zone of inhibition using Disc diffusion method of two different batches of 10μg and 30μg of ciprofloxacin discs from the same manufacturer.The Propose HPLC method is simple, precise, accurate, robust, significant and suitable for determining the assessment of ciprofloxacin content variation in antimicrobial susceptibility test discs by liquid chromatography.

Keywords: High Performance Liquid Chromatography, Ciprofloxacin, Zone of inhibition, Minimum inhibitory concentration, S. aureus, E. coli.