Abstract

A COMPARATIVE STUDY OF BLOOD FILM, IMMUNOCHROMATOGRAPHIC TEST AND POLYMERASE CHAIN REACTION IN THE DIAGNOSIS PLASMODIUM VIVAX INFECTED PATIENTS IN KHARTOUM STATE SUDAN

Sara Hassan Omer Elsheikh, Nada Mohamed Khalifa, Nisreen Barakat Babiker, Estishad Abdo, Rahma Alfadil, Mohammed Madani, Hisham Ali Mohamed Waggiallah and Linda Bashier Eltayeb*

Abstract

Background: Malaria is a major public health problem in Sudan. It is the most important human parasitic disease throughout the tropical and subtropical regions due to it is high prevalence and mortality rate, blood film, ICT and PCR are laboratory method used for diagnosed of malaria parasite. Objectives: The aim of this study was to compare between three Methods (blood film, immunochromatographic test and PCR) used for detection of Plasmodium vivax among Sudanese patients with malaria. Methods: Cross sectional study was conducted during the period from March to April 2018, fifty samples were collected from known patients with malaria, blood film, ICT and PCR was used to detected of plasmodium species from each sample; Data were collected using structural questionnaire, analysis was carried out by means of statistical package for social science (SPSS version 21). Results: From fifty malaria samples caused by plasmodium species, there are fifty samples 48(96%) and 43 (%) were positive by blood film and ICT respectively, but by used PCR technique there was fifteen 15(30%) of patients samples was positive for plasmodium vivax and P. falciparum. Conclusion: The high number of false negatives in microscopy, it is necessary to reinforce training of laboratory staff on malaria microscopy, the “Gold Standard” in endemic areas.

Keywords: Malaria, P-vivax, BFFM, ICT, PCR and Sudan.