Abstract

INHIBITORY EFFECT OF ETHANOLIC EXTRACT OF CITRUS PARADISI AND NARINGIN ON LIPID ACCUMULATION IN 3T3-L1 CELL LINES

R. Roghini and K. Vijayalakshmi*

Abstract

Background: The aim of the study is to examine the effects of Naringin and ethanolic extract of Citrus paradisi on lipid accumulation in 3T3-L1 cell line. Materials & Methods: Various tests such as MTT assay, Lipid accumulation assay by oil red O staining, Cell proliferation assay, Measurement of free Triglycerides content, Glycerol 3 Phosphate dehydrogenase was assessed Mean standard deviation (SD). One-way analysis of variance (ANOVA) was used. The significance of differences between two groups (control and sample) was analyzed by the Student’s t-test. Results: Study findings show that the lipid accumulation was significantly reduced with all concentration of Citrus paradisi and Naringin compared with the control; Citrus paradisi and Naringin treatment on 3T3-L1 pre-adipocytes during proliferation decreased the cell viability in all treated groups was noted. Among the concentrations tested, 500μg/mL significantly reduced the viability of 3T3-L1 cells. There was a highly significant decrease in TG level at 250μg/ml, 500 μg/ml, and 750 μg/ml (P

Keywords: Citrus paradisi, Naringin, 3T3-L1 preadipocytes, GPDH activity, MTT assay.