RP-HPLC METHOD DEVELOPMENT AND VALIDATION OF NORFLOXACIN IN BULK FORM
Sharma Ravi*, Kaur Amandeep, Malhotra Dipali
Abstract
A simple, selective, accurate and precise high-performance liquid
chromatographic (HPLC) method for estimation of Norfloxacin in bulk
form was developed & validated. The estimation was carried out on
Phenomenax luna C-18 (250x4.6 mm, 5μ) column using a mobile
phase consisting of 0.03 M potassium dihydrogen phosphate,
triethyamine: Acetonitrile (88:12 v/v) of pH 3.8 adjusted with
orthophosphoric acid, at a flow rate 1 ml/min. The UV detection was
carried out at 280 nm. Method validation was performed as per the
ICH guidelines. The method was validated for the precision, accuracy,
linearity and robustness.
Keywords: RP-HPLC estimation, Validation and Norfloxacin
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