Abstract

FINGER PRINTING ANALYSIS OF THE STEROIDS FROM AILANTHUS EXCELSA (ROXB.) LEAVES USING HIGH-PERFORMANCE THIN LAYER CHROMATOGRAPHY

Amita Tilak*, Mudit Kumar, Ranjana Sharma and Sudhir Singh Gangwar

Abstract

Objective: The present study was conducted to identify the steroids from petroleum ether (PEAE) extract of medicinally and economically useful leaves of Ailanthus excelsa (Roxb.) using High Performance Thin Layer Chromatography (HPTLC) technique. Methods: Preliminary phytochemical screening was done and HPTLC studies were carried out. CAMAG HPTLC system equipped with Linomat V applicator (Switzerland). Densitometric scanning was performed with Camag TLC scanner IV in the reflectance absorbance mode at 540 nm and operated by Win CATS software (1.4.6 Camag) with the help of tungstant lamp. Results: HPTLC finger printing of steroids of petroleum ether extract of leaves revealed six polyvalent phytoconstituents (6 peaks) and corresponding ascending order of Rf values in the range of 0.559 to 0.919. Conclusions: With the results of HPTLC analysis and above Rf values we have concluded the presence of steroids in the extract.

Keywords: Ailanthus excelsa (Roxb.) leaves, steroids, HPTLC Fingerprinting.