Abstract

HPLC: A SIMPLE AND ADVANCE METHODS OF SEPARATION AND VALIDATION

Vare S. R.*, Shelke M. M., Bidkar J. S. and Dama G. Y.

Abstract

High performances liquid chromatography (HPLC) is an essential analytical tool in assessing drug in pharmaceutical product. The present study focuses on the various steps, parameter involved in HPLC condition. HPLC process development is important in case drug discovery, drug development and pharmaceutical product. Now a day reverse phase chromatography is most commonly used separation techniques in HPLC. Reversed phase chromatography has found both analytical and preparative application in the area of biochemical separation and purification. The article designing HPLC method development and validation.

Keywords: High performance liquid chromatography, formulation and Development, validation, chromatography, HPLC etc.