Abstract

ISOLATION AND OPTIMIZATION OF AMYLASE PRODUCING BACTERIA AND ACTINOMYCETES FROM SOIL SAMPLE

CH. Sowjanya*, D. Rayalamma, D. Rani, Esenjungla, G. Sadhana

Abstract

Amylases are amongst most widely used enzymes in industries such as food, fermentation, starch processing, textile and paper. The objective of the present study was to isolate, identify and optimize potential amylase producing bacteria and actinomycetes from soil samples. Soil is the ideal habitat for many extracellular enzyme producing bacteria. The soil samples were collected from Thaluru and Medikonduru of Guntur district. Isolation was done by serial dilution and spread plate method. Primary screening of amylolytic activity of the isolates was performed by starch agar plate method. The submerged state fermentation method followed for the production of amylase by the optimization of temperature, pH, fermentation time and substrate concentration. From the soil samples, 16 isolates were 3 identified and subjected to primary screening for amylolytic activity. Of which, four isolates were observed with maximum amylolytic activity during the primary screening. During the submerged state fermentation, maximum amylase activity was observed at 48 h and then declined. The optimum temperature observed for maximum amylase activity of Bacillus was 40°C and Streptomyces at 37°C. The highest amylase activity was observed at neutral pH and 4% of starch concentration.

Keywords: amylase, actinomycetes, optimization, isolation.