Abstract

INVITRO ANTIOXIDANT ACTIVITY AND ANTI -INFLAMMATORY ACTIVITY OF ETHANOLIC LEAF EXTRACT OF COCCULUS HIRSUTUS

Dr. S.Umamaheswari*, K.S. Sridevi Sangeetha

Abstract

Aim is to study the invitro antioxidant and anti-inflammatory action of the ethanolic extract of leaves of Cocculus hirsutus. Preliminary Phytochemical anlalysis showed the presence of flavones, flavonoids, triterpenoids, anthocyanins, coumarins, proteins, reducing sugars, alkaloids, tannins, glycosides and quinines. The invitro antioxidant study was done by DPPH, Nitric oxide radical generation and reducing power assay. The anti-inflammatory study was done by HRBC (Human Red Blood Cell) assay. Ethanolic extract of the leaf of the plant showed significant antioxidant activity and the anti inflammatory activity. The maximum percentage of inhibition by DPPH method was found to be 56.73 % at the concentration of 800 μg/ml and the maximum percentage of inhibition by Nitric oxide method was found be 58.6 %. The invitro anti inflammatoy activity was found to 79.77 % at the concentration of 25 μg and it increases with increase in concentration at the concentration of 800 μg it was found to be 94.74%. The antioxidant and the antinflammatory activity is may due to the presence of flavonoids. From the above it was concluded that the ethanolic leaf extrac of cocculus hirustus having in vitro antioxidant and anti-inflammatory activity. Further investigation has to be done in experimental animals to find out the mechanism of action.

Keywords: Cocculus hirsutus, antioxidant, HRBC, inflammation.