Abstract

IN-VITRO ANTIOXIDANT ACTIVITY AND FREE RADICAL SCAVENGING POTENTIAL OF ROOTS OF SALVIA SPLENDENS (SCARLET SAGE)

Sailesh Narayan*, Abhilasha Mittal

Abstract

Objective: The objective of present work is to study the In-vitro antioxidant activities of petroleum ether, ethyl acetate and methanolic extracts of Salvia Splendens roots. Material and Methods: The extracts were studied using 1,1-diphenyl-2-picryl hydrazyl (DPPH), Hydrogen peroxide (H2O2), Total Phenolic Content (TPC) and Total Flavanoid Content (TFC). The total Phenolic contents and Flavanoid contents were estimated taking gallic acid and rutin calibration curve respectably. Results: All the extracts posses In-vitro anti-oxidant activities. But the order of possessing activities were methanolic > ethyl acetate > petroleum ether extracts of Salvia Splendens roots. The total phenolic content (TPC) and total flavanoid content (TFC) was highest in Methanolic extract. Conclusion: It can be concluded that Salvia Splendens roots extracts possess anti-oxidant activities.. The methanolic extract of Salvia Splendens roots possess highest anti-oxidant activity in-vitro.

Keywords: Salvia Splendens, In-vitro anti-oxidant, 1,1-diphenyl-2-picryl hydrazyl (DPPH), Ferric Reducing Power Activity, H2O2 Scavanging , Total Phenolic Content (TPC), Total Flavanoid Content (TFC).