Abstract

EVALUATION OF IN VITRO ANTIOXIDANT CAPACITY AND REDUCING POTENTIAL OF POLYHERBAL FORMULATION OXIWELL: A NUTRITIONAL SUPPLEMENT

Poonam Bhadauriya* and Arvind S. Jadon

Abstract

In biological system, reactive oxygen species (ROS) and reactive nitrogen species (RNS), such as superoxide, hydroxyl, and nitric oxide radicals, can damage the DNA and lead to the oxidation of lipid and proteins in cells. Normally, antioxidant system occurring in human body can scavenge these radicals, which would keep the balance between oxidation and anti-oxidation. Nonetheless, the exposure of cigarette smoking, alcohol, radiation, or environmental toxins induces the production of excessive ROS and RNS, which disrupt the balance between oxidation and anti-oxidation and result in some chronic and degenerative diseases. Many herbs contain antioxidant compounds which protects the cells against the damaging effects of reactive oxygen species. Polyherbal formulations containing different herbs are used for the treatment of various diseases. The study revealed a very good in vitro free-radical scavenging properties of oxiwell a polyherbal formulation from ayurwell labs which contains the extracts of Mangifera indica, Withania somnifera, Glycyrrhiza glabra, Vitis vinifera, powders of Emblica officinalis; and oils of Triticum sativum. The antioxidant activity of this formulation was evaluated by measuring reducing ability, free radical scavenging activity by DPPH and hydrogen peroxide methods.

Keywords: Free radicals, antioxidants, polyherbals.