Abstract

EVALUATION AND COMPARISON OF DISINFECTANT ACTIVITY OF SOME COMMERCIAL BRANDS BY USING STANDARD METHODS

Samiksha P. Sondawale*, Yogesh N. Gholse, Rahul H. Kasliwal and Dinesh R. Chaple

Abstract

The aim of this study was to evaluate and compare some commercially available disinfectant for their efficacy at laboratory level. The commercially available brands of disinfectant i.e. Harpic and Patanjali was evaluated and compared for their efficacy. The three microorganisms namely Escherichia coli, Staphylococcus aureus and Bacillus subtilis were used to check the activity of the disinfectant depending upon the procedures. The efficacy of disinfectant were determined by standard procedures such as Phenol coefficient test, Rideal Walker test, Chick Martin test, Kelsey Skyes test, Disk Diffusion test, Serial Dilution test, Viable Count test. The effectiveness of the disinfectant were determined by their ability to reduce the microbial growth and activity. On the basis of results from the standard procedures the Harpic was found to be more effective than Patanjali as per our findings on laboratory scale.

Keywords: Disinfectant, Microbes, Efficacy, E.coli, Phenol coefficient test, Kelsey Skyes test.