Abstract

SEPARATION, PARTIAL PURIFICATION AND MITOGENICITY OF ENTERIC VIBRIO FLUVIALIS ANDAEROMONAS HYDROPHILA LIPOPOLYSACCHRIDES IN CHICKEN AND RAT

Dr.Ibrahim M S Shnawa*, Khalied Y Alzamily and Dr.Rabab Omran

Abstract

Crude smooth LPS preparations were made from Vibrio fluvialis and Aeromonas hydrophila. Then these preparations were processed in gel filteration using sepharose 50 resine which give two fractions from each of the test isolates that reach the limits of partial purity. Test for an in vivo mitogenicity were carried out using chicken and rat models. The assessments were performed through skin induration and bone marrow lymphocyte blastogenicity. Fraction 1 and fraction 2 from each of the LPS preparations were proved to be mitogenic, Though F2 of the two LPS preparations were of higher mitogenicity than fraction 1.Such mitogenic potentials were stable and crossing the species barriers.

Keywords: Crude, gel filteration ,purity ,LPS, induration, blastogenicity, mitogenicity.