Abstract

CYTOTOXIC AND IMMUNOMODULATION EFFECT OF ROASTED FLAXSEED AND SOYBEAN EXTRACTS

Dharshini, T.Thirunalasundari, S.Sumayaa

Abstract

Research is focusing on the search for new types of natural chemotherapeutic agents derived from plant sources which are providing to be excellent sources of new compounds. Thus, Soybean (Glycine max (L)) and flaxseed (Linuim usitatissimum L.) are known to have a number of health benefits ranging from the well documented many chronic diseases. The present research article was aimed to study the cell viability and cytotoxic activity of roasted form of soybean and flaxseed extracts. The in-vitro methods used for the determination of cell viability by trypan blue dye exclusion method and cytotoxicity by MTT and SRB assay against MCF-7. The cell viability also showed significant result.The IC50 value of roasted form of soybean and flaxseed are 125μg & 80μg, 135 μg & 140μg respectively in MTT and SRB assay whereas this extracts were found to be non-toxic to normal PBMC. It is proved that the extracts of roasted soybean and flaxseed exhibits significant anticancer potential non-toxicity on MCF-7 cell line.

Keywords: soybean, flaxseed, MTT, SRB, PBMC and MCF-7 cell line.