Abstract

IN-VITRO ANTILEPROTIC ACTIVITY OF PETROLEUM ETHER EXTRACTS OF DALBERGIA SISSOO BARK

Ghogare Pradip Babasaheb* and Bhambar Rajendra Sudhakar

Abstract

Leprosy is a chronic infectious disease mainly affecting skin and peripheral nerves in patients. The present study investigated activity of ethanolic extracts of Dalbergia sissoo bark against Mycobacterium leprae using agar-well diffusion method. Zone of inhibition was determined by taking Dapsone as the positive control and ethanol as the negative control. Dalbergia sissoo was found effective against M. leprae. Material and Method: Plant material: Dalbergia sissoo Bark was collected from riverside of Loni KD. Dist. Ahmednagar (Maharashtra). The taxonomical identification and authentication was done by Dr. Medakkar Head department Botany Arts, Science and commerce college Rahuri. Test Organism: The test microorganism used in the study was Mycobacterium leprae. The bacterial strain was maintained in sterile conditions and was grown on nutrient agar medium. Standard bacterial suspension of 108 (CFU)/ml was prepared and used. Preparation of extracts: Bark of plant of Dalbergia sissoo was washed with distilled water further it was separated and air dried at room temperature under shade. 250 gm of the dried bark was extracted with Petroleum ether by soxhlation method. Phytochemical investigation of the extracts was conducted. These extracts were used to study the in-vitro antileprotic activity. Method: Cup-plate agar diffusion method using Nutrient agar: In a radial or 2D technique, Petri dishes of agar were prepared by pouring melted agar media previously inoculated with Mycobacterium Leprae microorganism. After the solidification agar cups are made with the help of borer and cups are filled with solution of suitable concentration of sample and standard respectively and are inoculated at 370C for 24 hrs. Result and Discussion: Petroleum ether extract of Dalbergia sissoo bark was used to determine its antileprotic activity via an Agar diffusion method. The Antileprotic activity ranges from 4.55 mm to 6.22 mm for Mycobacterium Leprae in comparison with std. Antileprotic drug Dapson (100 μg /ml); it was found that the Petroleum ether extract of β-Sitosterol (100 μg /ml) has Antileprotic activity almost equivalent to standard compound. Conclusion: The Petroleum ether extracts shows the presence of β sitosterol as active compound. The Preliminary Phytochemical studies of this extracts shows the presence of steroids, Alkaloids, Flavonoids, Saponins, Tannin compounds. Which are responsible for Antileprotic activity.

Keywords: Dalbergia sissoo bark, Petroleum ether extracts, ? sitosterol, Mycobacterium Leprae.