IN-VITRO ANTILEPROTIC ACTIVITY OF PETROLEUM ETHER EXTRACTS OF DALBERGIA SISSOO BARK
Ghogare Pradip Babasaheb* and Bhambar Rajendra Sudhakar
Abstract
Leprosy is a chronic infectious disease mainly affecting skin and
peripheral nerves in patients. The present study investigated activity of
ethanolic extracts of Dalbergia sissoo bark against Mycobacterium
leprae using agar-well diffusion method. Zone of inhibition was
determined by taking Dapsone as the positive control and ethanol as
the negative control. Dalbergia sissoo was found effective against M.
leprae. Material and Method: Plant material: Dalbergia sissoo Bark
was collected from riverside of Loni KD. Dist. Ahmednagar
(Maharashtra). The taxonomical identification and authentication was
done by Dr. Medakkar Head department Botany Arts, Science and
commerce college Rahuri. Test Organism: The test microorganism
used in the study was Mycobacterium leprae. The bacterial strain was
maintained in sterile conditions and was grown on nutrient agar medium. Standard bacterial
suspension of 108 (CFU)/ml was prepared and used. Preparation of extracts: Bark of plant of
Dalbergia sissoo was washed with distilled water further it was separated and air dried at
room temperature under shade. 250 gm of the dried bark was extracted with Petroleum ether
by soxhlation method. Phytochemical investigation of the extracts was conducted. These
extracts were used to study the in-vitro antileprotic activity. Method: Cup-plate agar
diffusion method using Nutrient agar: In a radial or 2D technique, Petri dishes of agar were
prepared by pouring melted agar media previously inoculated with Mycobacterium Leprae
microorganism. After the solidification agar cups are made with the help of borer and cups
are filled with solution of suitable concentration of sample and standard respectively and are
inoculated at 370C for 24 hrs. Result and Discussion: Petroleum ether extract of Dalbergia sissoo bark was used to determine its antileprotic activity via an Agar diffusion method. The Antileprotic activity ranges from 4.55 mm to 6.22 mm for Mycobacterium Leprae in comparison with std. Antileprotic drug Dapson (100 μg /ml); it was found that the Petroleum ether extract of β-Sitosterol (100 μg /ml) has Antileprotic activity almost equivalent to standard compound. Conclusion: The Petroleum ether extracts shows the presence of β sitosterol as active compound. The Preliminary Phytochemical studies of this extracts shows the presence of steroids, Alkaloids, Flavonoids, Saponins, Tannin compounds. Which are responsible for Antileprotic activity.
Keywords: Dalbergia sissoo bark, Petroleum ether extracts, ? sitosterol, Mycobacterium Leprae.
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