Abstract

SIMULTANEOUS DETERMINATION AND VALIDATION OF HYDROCHLORTHIAZIDE & CANDESARTAN IN BULK DRUG AND MARKETED FORMULATION BY RP-HPLC

P. Omkar Naath*, Dr. P. Arun, P. Shailendra, P. Bhavesh and D. Neelesh

Abstract

The aim of this work is to develop simple and precise Reverse phase High performance liquid chromatography (RP-HPLC) method for simultaneous determination of Hydrochlorthiazide & candesartan using single method in bulk drug and marketed formulation. Hydrochlorothiazide in various solvents, it was found 50 Mm KH2PO4: Acetonitrile (30:70) best suitable mobile phase. The selection of separation variable like mobile phase composition, flow rate and detection wavelength. The study shows the system suitability parameter less S.D. value shows good result and the result of linearity of Candesartan it was found 3-6 μg/ml and the standard calibration curve of Candesartan regression equation was determined and R2 was calculated and it was found to be 0.999 so it is linear between the concentration range 8-40 μg/ml. The study shows the result of linearity of Hydrochlorothiazide it was found 5-25 μg/ml.

Keywords: Hydrochlorthiazide, Candesartan, RP-HPLC, Acetonitrile, Chromatography, Linearity.