Abstract

ASSAY OF CIPROFLOXACIN BY ULTRAVIOLET-VISIBLE SPECTROSCOPY

*Ronald Bartzatt

Abstract

Ciprofloxacin belongs to a group of drugs referred to as fluoroquinolone class antibiotics, and is utilized to treat bacterial infections. This antibiotic was assayed by ultraviolet-visible spectroscopy by use of single-beam SpectroVis spectrometer in onecentimeter plastic cuvettes at 390 nm. Samples were analyzed from normal saline, aqueous mixture of dextrin/cellulose/starch/lactose, and aqueous solvent having 1% (v/v) ethanol, 1% (v/v) acetic acid (0.167 molar), 98% (v/v) distilled water. The standard curve ranged from 1.3919 x 10-3 molar to 1.4934 x 10-2 molar. The equation of line for standard curve is: y = 52.388x + 0.1435, with coefficient of determination R2 of 0.9943, meaning 99.43% of the proportion of the variance in the dependent variable (absorbance) is predictable from the independent variable (concentration in molar). The Pearson r correlation coefficient is 0.9971. The limit of detection (LOD) is 1.0915 x 10-3 molar and the limit of quantitation (LOQ) is 3.6382 x 10-3 molar. The assay of this important broad-spectrum antibiotic that is active against both Gram-positive and Gramnegative bacteria is vital for manufacturing production assurance, patient compliance assurance, and prevention of adulteration of product.

Keywords: ciprofloxacin, UV/Vis spectroscopy, antibiotic, Spectro-Vis.