Abstract

SYNTHESIS OF SILVER NANOPARTICLES USING ORANGE PEEL EXTRACTS AND THEIR ANTIBACTERIAL ACTIVITY

Dr. S. Usharani* and B. Ruba Devi

Abstract

Green synthesis of metallic nanoparticles has gained significant attention in the field of nanomedicine as an environment-friendly and cost-effective alternative in comparison with other physical and chemical methods. Several metals such as silver, gold, iron, titanium, zinc, magnesium and copper have been subjected to nanoformulation for a wide range of useful applications. Silver nanoparticles (AgNPs) are playing a major role in the field of nanomedicine and nanotechnology. They are widely used in diagnostics, therapeutic and pharmaceutical industries. Studies have shown potential inhibitory antimicrobial, anti-inflammatory and antiangiogenesis activities of AgNPs. AgNPs have been synthesized using silver nitrate and methanolic root extract of orange peel that belongs to the Rutaceae family. Synthesized nanoparticles were characterized by Fourier transforms infrared spectroscopy. Furthermore, the antibacterial effect of the plant extract and the nanoparticles were evaluated against gram-positive (Bacillus subtilis) and gram-negative (Escherichia coli) bacteria. The average size of AgNPs synthesized, was 20 nm with the spherical shape. Orange peel extract based nanoparticles exhibited improved antibacterial activity against both gram-positive and negative strains.

Keywords: FT-IR, Orange peel extract, SNP, Antibacterial activity.