Abstract

EVALUATION OF ENZYMATIC AND NON ENZYMATIC ANTIOXIDANT ACTIVITY OF ARISTOLOCHIA BRACTEATA RETZ

Muthuraj K*, K. Armugasamy, N Nagarajan, P. Sumathi and E. Krishnan

Abstract

This study significantly focused on the enzymatic and non enzymatic antioxidant activity of Aristolochia bracteata Retz. belongs to the family Aristolochia ceae which is exploited for the treatment of various free radical mediated ailments. Plant is mainly used for antidote and skin disease. Catalase activity, Superoxide dismutase, reduced Glutathione, Ascorbic acid, α-Tocopherol and total Carotenoids were estimated by following methods. The enzymatic assay shows best activity in Catalase activity (28.3±0.14 μ/mg), reduced Glutathione (49.7±0.08 μ/mg) followed by Superoxide dismutase (17.8±0.25 μ/mg) respectively and in non enzymatic assay Carotenoids (283.8±6.14mg/g) shows significant results. Thus this plant can be suggested as an alternative source for antioxidants.

Keywords: Enzymatic, Non-Enzymatic, Aristolochia bracteata.