Abstract

STANDARDISATION OF KALYANAKA GHRITA AND KSHEERAKALYANAKA GHRITA BY HIGH PERFORMANCE THIN LAYER CHROMATOGRAPHY

Dr. Aparna Nandakumar*, Dr. Ashok Kumar B. N., Dr. Ravindra Angadi, Dr. R. R. Geethesh and Dr. Vidyanand Mohan

Abstract

Background: Kalyanaka Ghrita is a formulation which has been mentioned in various classical compendium of Ayurveda like Charaka Samhita, Sushruta Samhita, Ashtanga Hrudaya etc. According to the reference in Charaka Samhita, it is a poly herbal preparation comprising of 28 drugs. Along with the reference of Kalyanaka Ghrita, Acharya Chakrapanidatta has quoted the reference of Ksheerakalyanaka Ghrita. The only difference between both the formulations is the ratio of water and the addition of milk in case of Ksheerakalyanaka Ghrita. Aims and Objectives: To compare, standardise and evaluate the different constituents present in Kalyanaka Ghrita and Ksheerakalyanaka Ghrita. Methodology: 4μl and 8μl of the above sample of Kalyanaka ghritha and Ksheerakalyanaka ghritha was applied on a precoated silica gel F254 on aluminum plates to a band width of 8 mm using Linomat 5 TLC applicator. The plate was developed in Toluene – Ethyl acetate (9:1) and the developed plates were visualized under UV 254 and 366 nm, and after derivatisation in vanillin-sulphuric acid spray reagent and scanned under UV 254 and 366 nm. Rf, colour of the spots and densitometric scan were recorded. Results: Under Short UV, 2 and 3 peaks were observed for Kalyanaka Ghrita and Ksheerakalyanaka Ghrita respectively.4 Peaks were observed under long UV for Kalyanaka Ghrita and 3 peaks were observed for Ksheerakalyanaka Ghrita. Under post derivatisation, 6 peaks were observed for Kalyanaka Ghrita and 7 peaks were observed for Ksheerakalyanaka Ghrita. Conclusion: The peaks observed in HPTLC can be considered for fingerprint profile analysis.

Keywords: Kalyanaka Ghrita, Ksheera Kalyanaka Ghrita, HPTLC.