Abstract

SCREENING OF THERMOPHILIC BACTERIA PRODUCING LIPASE ENZYME IN GEOTHERMAL AREAS OF SIPOHOLON-TARUTUNG, NORTH SUMATRA, INDONESIA

Hadela Yuri Putri and Anthoni Agustien*

Abstract

Thermophilic microorganisms can synthesize stable molecules in hot conditions, including enzyme molecules. Thermophilic bacteria develop at high temperatures, grow in hot springs, desert lands, and spas. This study aimed to obtain and isolate thermophilic bacteria that produce lipase enzyme and to know the macroscopic and microscopic thermophilic bacteria that have lipolytic potential. Hot water samples were obtained from the Sipoholon-Tarutung Hot Springs, North Sumatra, Indonesia and then recorded the physical-chemical form and bacterial characterization of these hot springs. Eleven bacteria were selected based on differences in morphological characteristics, seven of which could produce lipase enzymes and carry out macroscopic and microscopic characterizations. BTPL-01 is the isolate which has the highest protease activity because the proteolytic index value is 0.85.

Keywords: Bacteria, Enzymes, Hot Springs, Isolation, Screening, Thermophilic.