Abstract

ANTIGLYCATION AND ANTIOXIDANT ACTIVITY OF A HOMEOPATHIC TINCTURE USED IN SRI LANKA

Hiniduma Vithanachchige Nadeesha Lakmali, Srianthie A. Deraniyagala* and Chamira Dilanka Fernando

Abstract

Glycation is the non-enzymatic reaction between a protein and sugars. After a series of complex reactions following glycation, advanced glycation end products (AGEs) are formed. AGEs are responsible for the pathogenesis of diabetic complications. Among the alternative treatments in Sri Lanka, the mother tincture of Syzygium jambolanum (SJ) is a widely used in homeopathy for treating diabetes mellitus. This study investigates the inhibitory potential on AGE formation by SJ and also its antioxidant activity. Nonenzymatic protein glycation inhibitory capacity assessed by Bovine Serum Albumin-Fructose fluorescence spectrometric assay showed that SJ at concentrations 4.2-25.2 mg/ml, inhibited AGE formation by 76.7±3.7%-95±0.9%. The positive control aminoguanidine at concentrations of 0.5-5.0 mg/mL, inhibited 65±3.5%-98±1.0%. The total phenolic content of SJ was 114.8±1.8 mg GAE/g in the Folin Ciocalteu assay. The percentage radical scavenging activity observed from DPPH assay for SJ (1.0-5.0 mg/ml) was 22.4±2.2%-87.7±1.0%, compared to the Ascorbic acid (ASC), positive control (0.5- 2.5 mg/ml) showing 31.9.0±0.4%-95.2±0.3%. Percentage hydroxyl radical scavenging activity observed for SJ (1.0- 5.0 mg/ml) was 23.7±0.4%-77.1±0.5% compared to ascorbic acid (0.1- 2.5 mg/ml) showing 22.8±0.5% - 97.5±1.1%. Our results indicate the mother tincture of SJ can prevent AGE formation and there by prevent the onset of diabetic complications. It also showed promising radical scavenging activity thus making it a potential remedy for many diseases in which radicals are implicated.

Keywords: Antiglycation potential, antioxidant capacity, homeopathy mother tincture Syzygium jambolanum.