Abstract

ADA ESTIMATION IN BODY FLUIDS OF TUBERCULOSIS PATIENTS

*Dr. Aparna Pandey PhD, Dr. Jayesh Prabhakar Warade MBBS MD

Abstract

Aim: The aim of the study is to determine the ADA level in tuberculous patient and figuring cut off value for ADA to use it as a diagnostic marker. Methods: ADA levels were estimated in serum and body fluids of 100 tuberculous and 100 non-tuberculous diseased patients. Results: Serum ADA for tubeculous patients (19.32±2.15 U/L) is significantly higher than non- tuberculous patients (10.55±0.90 U/L). Also the ADA in Pleural fluid (29.61±3.93), Pericardial Fluid (28.53±1.02), Peritoneal Fluid (27.91±2.23), CSF(28.40±1.86) in tuberculous patient is significantly higher than non tuberculous diseased pleural (10.33±1.28), peritoneal (10.11±0.53), pericardial (10.46±1.00) and CSF (10.07±0.88) . The cut off value suggested is 15 U/L which yelds the sensitivity and specificity of about 98% and 100 % respectively. The ratio of body fluid ADA to serum ADA in subjects with tubercular cases is significantly higher than and non-tubercular disease patients. Conclusion: Conventional markers of TB i.e. serum ADA levels are significantly higher in tuberculous patients as compared with the healthy subjects and subjects with other disease. It is also concluded from the study that the Body fluid (pleural fluid, pericardial fluid, peritoneal fluid and CSF) ADA to serum ADA ratio is significantly higher in TB cases as compared to that with non-tubercular cases. The cut-off value suggested for the parameters studied in the study yields a high sensitivity and specificity for the diagnosis of tubercular infection.

Keywords: Mycobacteria, pleural, ascites, parapneummonic, monocytes, peritonitis, empyema, meninigits.