Abstract

MODULATIONS IN UTERINE LIPIDS AND GLYCOGEN UNDER THE INFLUENCE OF CDRI-85/287 DURING PRE-IMPLANTATION PERIOD IN RAT: A HISTOCHEMICAL STUDY

K. Singh, F. W. Bansode* and J. D. Dhar

Abstract

In early pregnancy in rat, uterine lipids play a very vital role in the process of implantation especially for nutrition of blastocyst and in energy metabolism required for uterine proliferation. Thus present study was undertaken to study changes in histochemical localization of uterine lipids and glycogen under the influence of CDRI-85/287- a potent estrogen antagonist/antiimplantation agent in rat. Results revealed moderate staining intensity of uterine Red oil O-lipids and glycogen in uterine luminal epithelium on day 3 post-coitum (p. c.). While the stromal cells and glandular epithelium showed no lipid staining, intense staining of glycogen was noted in sub-epithelial stroma on day 3. On day 4 p.c. a marked increase in uterine (stroma/epithelium) lipids and glycogen staining reaction was observed. But, staining intensity for both lipids and glycogen on the antimesometrial side of uterus showed a decrease on day 5 p.c. as compared to day 4. Rats administered CDRI- 85/287 at its contraceptive dose (2.5 mg/kg) on day 1 p. c. showed a significant increase in staining intensity of lipids in uterine stroma (particularly in leucocytic cells) and blood vessels on day 3 as compared to controls. But, exhibited decreased staining intensity in uterine luminal epithelium from days 3-5. However, uterine glycogen staining reaction showed a significant decrease in treated rats from days 3-5. Results of this study indicate inhibition in uterine lipids and glycogen content during pre-implantation period under the influence of CDRI 85/287, thus contributing to the potency of this novel molecule as an antiimplantation and anti-estrogenic agent. Findings are discussed in relation to role of glycogen and lipids during the process of implantation in rat.

Keywords: Implantation-lipids-glycogen-85/287.