Abstract

ISOLATION AND SCREENING OF THERMOPHILIC BACTERIA PRODUCING AMYLASE FROM GEOTHERMAL REGION SIPOHOLON-TARUTUNG, NORTH SUMATRA, INDONESIA

Armila Nasution, Anthoni Agustien* and Feskaharny Alamsjah

Abstract

Thermophilic bacteria have an important role in the field of biotechnology because thermophilic enzymes are absorbed in large amounts in nature and have high stability due to changes in the temperature of their environment. This research on isolation and screening of themophilic bacteria aims to find thermophilic bacteria as a produce of amylase enzymes. The sample spring water ware obtained from sipoholon-tarutung hot sprong, north sumatera, Indonesia and then recorded the physical, chemical, form and bacterial characterization of Sipoholon-Tarutung hot springs. Ten bacteria were selected based on differences in morphological caracteristics, five of wich had the ability tapi produce amylase enzymes and carry put microscopic and macroscopic characterization. The isolate BTSA-01 which has the high amylolytic index value is 2.3 and the potential produce amylase enzymes.

Keywords: Isolation, Screening, Thermophilic Bacteria, Amylase, Hot Spring.