Abstract

AN EXPERIMENTAL STUDY TO EVALUATE THE ANTI-EPILEPTIC ACTIVITY OF KSHEERAKALYANAKA GHRITA BY PTZ INDUCED KINDLING AND STAGING METHOD

Dr. Aparna Nandakumar*, Dr. Ashok Kumar B. N., Dr. Ravindra Angadi, Dr. R. R. Geethesh and Dr. Vidyanand Mohan

Abstract

Kalyanaka Ghrita[1] is widely used to treat conditions like Unmada, Apasmara etc. Along with the reference of Kalyanaka Ghrita, Acharya Chakrapanidatta has quoted the reference of Ksheerakalyanaka Ghrita.[2] The ingredients of Kalyanaka Ghrita and Ksheerakalyanaka Ghrita is the same except for the addition of four parts of milk and two parts of water to it. Gokshira[3] is said to possess karmas like Rasayana, Medhya, Balya and Alakshmighna according to classical references; which is considered very essential in chiravyadhi like Apasmara.[4] Aims and Objectives: To evaluate the Antiepileptic activity of Ksheerakalyanaka Ghrita by PTZ Induced Kindling method. Methodology: For kindling induction, Pentylenetetrazol (PTZ) was freshly dissolved in normal saline and a sub-convulsive dose (35 or 40 mg/kg, ip) was administered every other day for a total of around 12 injections (which would in normal control mice induce convulsion of maximum intensity of grade 5). After each injection of sub-convulsive dose of PTZ, mice in different groups were observed for 30 min and PTZ-induced seizures were evaluated and classified according to the scoring system of Fischer and Kittner -1998. Data was expressed as mean ± SEM and the data was analysed by one way ANOVA followed by Dunnet’s Multiple‘t’ test as a post HOC test for parametric data. After Kindling, the brains were excised and subjected to Histopathological studies. Results: Ksheerakalyanaka Ghrita showed statistically non-significant improvement in PTZ induced Kindling study. Histopathological reports depicted the protective action on brain tissues. Conclusion: Ksheerakalyanaka Ghrita can be advised to cope up with the side effects of commonly prescribed A.E.Ds as it imparts a protective action on brain tissues.

Keywords: Ksheerakalyanaka Ghrita, Kindling, PTZ, Antiepileptic activity.