Abstract

QUANTITATIVE ANALYSIS AND IN VITRO FREE RADICAL SCAVENGING ACTIVITY OF CAYRATIA TRIFOLIA (L.)

Sundaram Sowmya, Palanisamy Chella Perumal, Palanirajan Anusooriya ,Balasubramanian Vidya Prabhakaran Pratibha and
Velliyur Kanniappan Gopalakrishnan1, 2, *

Abstract

Cellular damage caused by reactive oxygen species has been implicated in several diseases and hence antioxidants have significant importance in human health. The present study was to evaluate the quantitative analysis and in vitro free radical scavenging activity of stem ethanolic extract of Cayratia trifolia. The content of total phenols, tannins, saponins, flavonoids, alkaloids analyzed. Free radical activity was assayed using 1,1-diphenyl-2-picryl hydroxyl (DPPH), 2,2’ azinobis-3 ethylbenzothiozoline-6 sulfonic acid (ABTS+) cation decolourization test, hydroxyl radical (OH.), hydrogen peroxide assay (H2O2), nitric oxide radical inhibition activity (NO), superoxide radical, metal chelating assay, ferric reducing antioxidant power assay (FRAP), reducing power activity using established assay procedure. The ethanolic extract of Cayratia trifolia exhibited major content of secondary metabolites present in the stem extract. The antioxidant activity of the extract was compared with standard ascorbic acid. In conclusion, the results presented in the stem of Cayratia trifolia have a strong antioxidant property against free radicals and it may serve as a good pharmacological property.

Keywords: Cayratia trifolia, Ethanolic extract, Quantitative analysis, Free radical scavenging activity.