Abstract

INTERPRETATIVE IN VITRO PHYTOCHEMICAL,TLC, SYNTHESIS OF SILVER NANOPARTICLES AND THEIR ANTIBACTERIAL SCREENING OF AQUEOUS AND ETHANOLIC EXTRACTS OF MORINDA CITRIFOLIA L. (NONI) FRUIT AND THEIR COMPARATIVE STUDY

Jahnavi Alwala, Manisha D.R, Vasanth Kumar Mandla, Sreedhar Bojja, Sreelakshmi Chittamuru, Raju Nalvothula, M.P.Pratap Rudra*

Abstract

NONI is common name for Morinda citrifolia fruit. This belongs to Rubiacea family.Noni’s unique medicinal property is known to mankind since more than 2000 years.Noni helps in normalization of abnormally functioning cells by delivering the bio-chemical nutrients. It contains Proxeronine and more than 160 nutraceuticals.NONI is beneficial for the unique food supplement of which will be added to the probable list of drugs for cancer, heart diseases (hypertension), arthritis,allergy,menustral irregularities, gastric ulcers, diabetes, immune system, eye inflammations and it is a most important nutritional booster etc.,.The major aim of present study was to evaluate extractability of the aqueous and ethanolic extracts were assessed by the ecofriendly method and also to screen the ethno medicinal use, the phytochemical screening of Morinda citrifolia L.,(Noni fruit) excavate that most of the important constituents like alkaloids, glycosides, carbohydrates, flavonoids, terpenes, saponins, phenols, tannins, quinones, celluloses, steroids and gums which interprets its medicinal values. Biosynthesis of silver nanoparticles is an important in the field of nanotechnology, which has the economic and ecofriendly beneficial for chemical and biophysical characterization. The present study reveals the synthesis of silver nanoparticles using both the aqueous and ethanolic extracts of Morinda citrifolia fresh fruits were assessed by 1mM Silver nitrate solution. The synthesized nanoparticles werecharacterized by UV-Visible Spectrophotometer, Transmission Electron Microscopy (TEM), Fourier Transform Infrared (FTIR), Atomic Force Microscopy (AFM), also thin layer chromatography (TLC) for chromatogram visualization (Rf values) of both the aqueous and ethanolic extracts of Morinda citrifolia fresh fruits and also antibacterial studies for the formed nanoparticles.

Keywords: Morinda citrifolia, UV, TEM, FTIR, AFM.