Abstract

IDENTIFICATION OF LUPEOL DERIVED FROM IN VITRO CALLUS CULTURE OF HEMIDESMUS INDICUS (L.) R.BR. (ANANTAMOOL)

*Dr. Prasanna Purohit

Abstract

Hemidesmus indicus (L.) R. Br. is very useful herb in pharmacology. It is used a lot due to its medicinal properties. There are many drug preparations perform by using this plant. Many phytochemicals are present in root, leave and stem of Hemidesmus indicus (L.) R. Br. An efficient medium for callus formation was developed in MS and B5 media supplemented with 2, 4-D, NAA, BA and kinetin. The maximum percentage of callus induction was observed in MS medium containing 1.0 mg/l NAA and 2.0 mg/l KN for in vitro biosynthesis of antioxidants such as lupeol, from Hemidesmus indicus (L.) R.Br. (Anantamool) cultures. Used of identification method is Thin layer chromatography (TLC). In result of Thin layer chromatography analysis, Rf value (Retardation factor) of standard and that of sample extracts lies almost in same range which confirms that extracts contain is Lupeol.

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