Abstract

EVALUATION OF DIURETIC ACTIVITY OF HYDROCHLOROTHIAZIDE - ?-CYCLODEXTRIN INCLUSION COMPLEX IN WISTAR RATS

Sanae Masmoudi*, Bouchra Meddah, Mounia Bouabdellah  , Yahia Cherrah,Layachi Chabraoui , Abdelaziz Bouklouze

Abstract

The thiazide diuretics are the first line treatment recommended for hypertension, in particular hydrochlorothiazide (HCT). The aim of the present study is to evaluate the diuretic activity of HCT- γ- Cyclodextrin (HCT-γCD) inclusion complex in comparison with HCT alone. Vehicle (distilled water), HCT- γCD complex (10mg/kg) and HCT alone (10mg/kg) were administered orally to male wistar rats and their urine output was quantitated after 24 hours. Volume, pH, conductivity, and Na+, Cl- , K+ concentrations of urine were measured. The results showed significant improvement of the urine output of HCT-γCD complex compared to HCT alone respectively 5,52 ± 0.52 versus 4,3 ± 0.28 ml/100g/24h . Urinary electrolyte excretion and conductivity was also incresead. Significant diuretic (p < 0.05) and electrolyte excretion effects (p < 0.05) were observed in treated groups by HCT-γCD complex compared to the control and reference standard (hydrochlorothiazide). Therefore, we can conclude that HCT-γCD complex produced notable diuretic effect.

Keywords: Hydrochlorothiazide, ?- cyclodextrin, diuretic activity.